EN 13727 Chemical Disinfectants and Antiseptics: Quantitative Suspension Test for the Evaluation of Bactericidal Activity for Instruments Used in the Medical Area

Summary of Test

The EN method is designed to evaluate the bactericidal, fungicidal, yeasticidal, basic sporicidal or mycobactericidal activity of a product used under various conditions. Many methods are suspension based and are used to support general antimicrobial claims while other test methods are carrier-based and are used to support antimicrobial activity of products on surfaces or devices. In either case, the same general principles apply. The test organism is exposed to sample of the product in a manner which simulates the desired claim. Following exposure, the test system is neutralized and quantitatively assayed for survivors. The resulting plates are incubated, enumerated, and a reduction in viability or microbiocidal effect is determined as compared to a population control.

Test Organisms Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus hirae
Purpose of Test Suspension-based study formally used to evaluate bactericidal activity of products that are used in the medical area (e.g. hygienic handrub, hygienic handwash, surgical handrub, surgical handwash, instrument disinfection etc.)
Typical Performance Criteria
(Requirements may vary by claim)
3-5 log reduction in 1-5 minutes depending on claim