ASTM E2274 and E2406 Standard Test Method for the Evaluation of Laundry Sanitizers

Purpose of Test

The ASTM E2274 and E2406 Standard Test Methods are carrier-based test methods used to evaluate the sanitizing properties of laundry detergents/additives designed for top-loading and front-loading washing machine operations, respectively. These products are typically registered with the U.S. EPA.

Summary of Test

In this method, a series of fabric swatches are inoculated with the test organism and are dried. The swatches containing the dried film of test organism are placed inside fabric-wrapped spindles and are treated in a simulated laundry apparatus. Following treatment, the swatches and a sample of the wash water are removed and are assayed for surviving test organism. The resulting plates are incubated, the survivors are enumerated and a percent reduction is determined on the test carriers as compared to a population control. In order to successfully demonstrate sanitizing efficacy, a 99.9% reduction of the test organism must be demonstrated on the treated fabric swatches and in the treated wash water as compared to the population control. Typical test organisms include Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae.