ASTM E2180 Standard Test Method for Determining Antimicrobial Activity in Polymeric or Hydrophobic Materials

Purpose of Test

The ASTM E2180 Standard Test Method is used to evaluate the effectiveness of antimicrobial agents incorporated into/onto hydrophobic surfaces, commonly plastics or polymers. This method is generally used to substantiate antimicrobial properties of treated materials for non-public health related claims.

Summary of Test

In this method, treated test samples are inoculated with the test organism mixed with in a semi-solid agar “slurry” to facilitate surface interaction. The test organism is allowed to expose on the surface of the treated material typically for 24 hours. Following exposure, the sample is neutralized, the surface is washed and the sample is quantitatively assayed for survivors. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test sample as compared to the untreated control sample. No performance criteria currently exist for general antimicrobial claims made using this method. Common test organisms utilized in this method include Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae.