ASTM E1053 Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces

Purpose of Test

The ASTM E1053 test method is used to evaluate the efficacy of water soluble powders, liquids, or spray disinfectants as a virucide for hard, non-porous surfaces for registration with regulatory agencies such as the U.S. EPA and Health Canada. This method is intended to simulate and evaluate products intended for mop, soak, or spray based applications. This test method may be performed using a wide variety of human and veterinary viruses.

Summary of Test

A glass Petri dish (“carrier”) is inoculated with a representative test virus and the virus is dried onto the carrier. The carrier is inoculated with an aliquot of the use dilution of the test substance (liquid products), or to the amount of spray released under use conditions (spray products). The inoculated carrier is held for the requested exposure time at the requested exposure temperature. Following exposure, the contents of the carrier are neutralized and serial dilutions of the neutralized test substance are performed. The dilutions are then assayed for viral infectivity by an assay method specific for the test virus. Appropriate virus, test substance cytotoxicity, and neutralization controls are run concurrently. Typically, most regulatory agencies require complete inactivation of the virus in all dilutions assayed.

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