AOAC Method 966.04 Sporicidal Activity of Disinfectants

Purpose of Test

The AOAC Sporicidal Activity of Disinfectants test method is a carrier-based method used to evaluate water soluble powders and non-volatile liquid products for chemosterilant or sporicidal efficacy for registration with regulatory agencies such as the U.S. EPA, Health Canada and in the case of high-level disinfectants, the U.S. FDA.

Summary of Test

In this method, a series of porcelain cylinders and silk or Dacron sutures (“carriers”) are inoculated with the spores of Bacillus subtilis and Clostridium sporogenes and are desiccated. The carriers containing the dried spore film are then sequentially immersed into 10 mL of disinfectant and are exposed to the disinfectant for a pre-determined exposure time. After exposure, the carriers are sequentially transferred to a liquid subculture medium specifically selected to neutralize the test substance active and to recover any surviving spores. The carriers are incubated and visually examined for the presence or absence of growth. Based on the desired claim and the requirements of the regulatory agency, the product must demonstrate kill on a pre-determined number of carriers inoculated with test organisms applicable to the claim.