AOAC Method 960.09 Germicidal and Detergent Sanitizing Action of Disinfectants

Purpose of Test

The AOAC Germicidal and Detergent Sanitizing Action of Disinfectants test method is a suspension-based test method used to evaluate non-halide based water soluble powders and liquid products intended for the treatment of previously cleaned, nonporous, food contact surfaces (e.g., eating and drinking utensils and food processing equipment) as a terminal sanitizing rinse. These products are typically registered with the U.S. EPA.

Summary of Test

In this method, a sample of the disinfectant is inoculated with a suspension of representative test organism. Following a 30 second exposure, the sample is neutralized and quantitatively assayed for survivors. The resulting plates are incubated, the number of survivors is enumerated and a percent and log10 reduction is determined as compared to a population control. In order to successfully demonstrate sanitizing efficacy, the product must demonstrate a 99.999% (or 5 log10) reduction. Typical test organisms include Staphylococcus aureus and Escherichia coli. Additional food-borne pathogens of interest are often tested as well.