AOAC Method 955.17 Fungicidal Activity Method

Purpose of Test

The AOAC Fungicidal Activity Method is a suspension-based test method used to evaluate water soluble powders and non-volatile liquid products for fungicidal efficacy for registration with regulatory agencies such as the U.S. EPA and Health Canada. This method is intended to simulate and evaluate products intended for mop or soak-based applications.

Summary of Test

In this method, a sample of the disinfectant is inoculated with a suspension of fungi (Trichophyton interdigitale) and the suspension of fungi is then exposed to the disinfectant for 5 minutes, 10 minutes and 15 minutes. Following exposure, a sample of the exposed fungi is removed and is added to a tube containing a liquid subculture medium specifically selected to neutralize the test substance active and to recover any surviving fungi. The subculture tubes are incubated and visually examined for the presence or absence of growth. For disinfection claims, no growth must be observed after 10 minutes of exposure.